TSH Test procedure: TSH hormone test, Normal Value

TSH TEST PROCEDURE:

Method: ELISA (Enzyme linked immunosorbent assay)

Origin: Human GmbH, Germany

Working reagent Preparation:

Working wash solution: 1000ml with 50ml wash solution

Add distilled water and make a working wash solution in a clean bottle.

Or, as required, mix 1000ml distilled water with 5ml wash solution to make 105ml working wash solution.

Calibrators: A-0, B-0.50, C-3.0, D-6.0, E-15.0, F = 30.0

Pipetting scheme:

Reagent and Sample should be brought to room temperature before use

Sample / control / calibrators should be given in each well with the required number of wells.	50ul
Enzyme conjugate should be given in each well.	100ul
Mix and cover with adhesive strip
Keep at room temperature for 60 minutes.
Wash 5 times with working wash solution	300ul
Substrate solution n should be given in each well.	100ul
Keep room temperature for 15 minutes.
You have to give stop solution in each well.	100ul
You have to mix well.
With the help of 450nm filter it is  necessary to absorb quickly (within 10 minutes) or determine the result directly with ELISA reader.

Expected values: Normal range: 0.4- 6.2 miu/l